When using an acne spot treatment, such as La Roche-Posay Effaclar Duo Acne Spot Treatment, it is recommended to apply the treatment before your moisturizer. This allows the potent ingredients, such as 5.5% Micronized Benzoyl Peroxide and Lipo-Hydroxy Acid, to directly target the acne blemishes and work more effectively. After applying the spot treatment, wait for it to be absorbed into the skin, and then follow up with a moisturizer, like La Roche-Posay Effaclar Mat Mattifying Moisturizer for Oily Skin, to hydrate and protect your skin without causing additional breakouts.